Analysis to study the relationship between the chemical characterization of beers and the content of alpha acids present in two cultivars of hops

Authors

DOI:

https://doi.org/10.5965/223811712232023392

Keywords:

craft beer, antioxidants, Humulus lupulus L., brazilian hops

Abstract

Hops (Humulus lupulus L.) is a plant whose female inflorescence is used in the brewing industry to impart bitterness and aroma to the drink, and Brazil imports about 98% of this raw material. However, this plant has been gaining prominence in the country, directly influencing the quality characteristics of the final product. Thus, the aim of this study was to explore the relationship between the alpha acids content of two hop cultivars produced in Brazil and the United States. The work was developed at UDESC/CAV, in Lages/SC, in the year 2021. A standard Indian Pale Ale beer, with the same base recipe, was produced. Each treatment consisted of the addition of different hop cultivars (Comet and Fuggle) from two origins (Brazilian and American). The results confirm that the cultivars and the origin of the hops influence the chemical characteristics of the evaluated beers. The amount of total phenolic compounds, flavonoids and antioxidant activity of the beer produced with the Brazilian Fuggle cultivar was 6.5% (850.09 meq gallic acid L-1); 75.5% (95.07 meq quercetin L-1) and 13.8% (6890 mmol Trolox L-1) higher, respectively, in relation to beer produced with the same cultivar originating in the United States. There was an inverse correlation between the alpha acid content and the phenolic content and antioxidant capacity of the beers. The beers in which Brazilian Fuggle hops were used, with the lowest alpha acid content among those adopted, showed a greater positive association with the total polyphenol content, individuals and antioxidant capacity of the beers. In conclusion, the beers produced with the cultivar Fuggle Brasileiro have a greater concentration of antioxidant substances.

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References

ADAMS RP. 2007. Identification of essential oil components by gas chromatography/mass spectrometry. 4.ed. Carol Stream: Allured Publishing Corporation. 800 p.

ALONSO-ESTEBAN JI et al. 2019. Phenolic Composition and antioxidant, antimicrobial and cytotoxic properties of hop (Humulus lupulus L.) seeds. Industrial Crops and Products 134: 154–159.

ALVES V et al. 2020. Beer volatile fingerprinting at different brewing steps. Food Chemistry 326: 1-10.

ARRANZ S et al. 2012. Wine, Beer, Alcohol and Polyphenols on Cardiovascular Disease and Cancer. Nutrients 4: 759-781.

BENUCCI I et al. 2021. Novel microencapsulated yeast for the primary fermentation of green beer: kinetic behavior, volatiles and sensory profile. Food Chemistry 340: 1-11.

CERMAK P et al. 2015. Inhibitory effects of fresh hops on Helicobacter pylori strains. Czech Journal of Food Sciences 33: 302-307.

COMEX. 2021. Portal do comércio exterior do Brasil. Exportação e Importação geral. Disponível em: http://comexstat.mdic.gov.br/pt/geral. Acesso em: 4 abr. 2021.

EBC. 2010. EUROPEAN BREWERY CONVENTION. Analytica-EBC. Germany: Nürnberg.

ELROD SM et al. 2017. High Phenolic Beer Inhibits Protein Glycation in Vitro. J. Am. Soc. Brew. Chem 75: 1–5.

ELROD SM et al. 2019. Relationship between Phenolic and Antioxidant Concentration of Humulus lupulus and Alpha Acid Content. Journal of the American Society of Brewing Chemists 77: 134-139.

GOMES FO et al. 2022. Advances in dry hopping for industrial brewing: a review. Food Science and Technology 42: 1-8.

GRANATO D et al. 2011. Characterization of Brazilian Lager and Brown Ale Beers Based on Color, Phenolic Compounds, and Antioxidant Activity Using Chemometrics. J. Sci. Food Agric 91: 563–571.

HALEY E et al. 2012. A Multicomponent UV Analysis of α- and β-Acids in Hops. Journal of Chemical Education 89: 117–120.

HOPGUIDE. 2022. Disponível em: file:///C:/Users/Margarete/Downloads/Hop_EN%20(1).pdf. Acesso em: 05 fev.2023

HORNER CE et al. 1972. Registration of Fuggle H Hop. Crop Science 12: 1714.

IHGC. 2020. IHB-Sortenliste. Hopfen-Rundschau. Disponível em: https://www.deutscher-hopfen.de/downloads/IHB-Sortenliste%202020.pdf. Acesso em: 10 ma. 2020.

KIM D et al. 2016. Phenols displaying tyrosinase inhibition from Humulus lupulus. Journal of Enzyme Inhibition and Medicinal. Chemistry 31: 742-747.

KRETZER SG & CREUZ A. 2022. Lúpulo no Brasil: Perspectivas e realidades. In: Diagnóstico da situação atual do cultivo de lúpulo no Brasil. Brasília: MAPA. p. 12-49.

KROFTA K et al. 2008. Antioxidant characteristics of hops and hop products. Journal of the Institute of Brewing, 114: 160–166.

LIU M et al. 2015. Pharmacological profile of xanthohumol a prenylated flavonoid from hops (H. lupulus). Molecules 20: 754-779.

MEDA A et al. 2005. Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chem 91: 571-577.

MIKYŠKA A & KROFTA K. 2012. Assessment of changes in hop resins and polyphenols during long-term storage. Journal of the Institute of Brewing 118: 269–279.

NICKERSON GB. 1986. Varietal Differences in the Proportions of Cohumulone, Adhumulone, and Humulone in Hops. J. Am. Soc. Brew. Chem 44: 91–94.

NTOURTOGLOU G et al. 2020. Pulsed electric field extraction of α and β-acids 137 from pellets of Humulus lupulus (Hop). Frontiers in Bioengineering and Biotechnology 8: 1-12.

QUIFER-RADA P et al. 2015. A Comprehensive Characterisation of Beer Polyphenols by High Resolution Mass Spectrometry (LC–ESI-LTQ-OrbitrapMS). Food Chemistry 169: 336-343.

R CORE TEAM. 2013. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna: Austria.

RUFINO MSM et al. 2006. Metodologia científica: determinação da atividade antioxidante total em frutas pelo método de redução do ferro (FRAP). Fortaleza: Embrapa. 4p.

SILVA MC et al. 2021. A Simple Method for Evaluating the Bioactive Phenolic Compounds’ Presence in Brazilian Craft Beers. Molecules 26: 1-16

SINGLETON VL & ROSSI J. 1965. Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. Am J Enol Vitic, 16: 144-158.

SIQUEIRA PB et al. 2008. O processo de fabricação da cerveja e seus efeitos na presença de polifenóis. Alimento e Nutrição 19: 491-498

SIRRINE R et al. 2010. Sustainable Hop Production in the Great Lakes Region. Extension Bulletin, 3083: 1-12.

VAN DEN DOOL H & KRATZ P. 1963. A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. Journal of Chromatography 11: 463–471.

ZHAO H et al. 2010. Phenolic profiles and antioxidant activities of commercial beers. Food Chemistry 119: 1150–1158.

Published

2023-08-04

How to Cite

ARRUDA, Ana Luiza; SOLDI, Cristian; AGOSTINI, Evelyn; RUFATO, Leo; RUFATO; KRETZSCHMAR, Aike Anneliese. Analysis to study the relationship between the chemical characterization of beers and the content of alpha acids present in two cultivars of hops. Revista de Ciências Agroveterinárias, Lages, v. 22, n. 3, p. 392–402, 2023. DOI: 10.5965/223811712232023392. Disponível em: https://periodicos.udesc.br/index.php/agroveterinaria/article/view/23111. Acesso em: 22 nov. 2024.

Issue

Section

Research Article - Science of Plants and Derived Products

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