Comparação da eficiência entre dois métodos de inseminação artificial utilizando sêmen fresco em gata doméstica (Felis Catus)
DOI:
https://doi.org/10.5965/223811712212023085Palavras-chave:
inseminação intra-uterina, técnicas de reprodução assistida, sêmen fresco, inseminação, gatosResumo
O objetivo deste estudo foi comparar as taxas de prenhez em gatas domésticas usando sêmen fresco para o método de inseminação intrauterina bilateral (BIUI) e o novo método de inseminação do corpo uterino (UBI). As gatas receberam uma única injeção de gonadotrofina coriônica equina (eCG) (200 UI; IM) para induzir o desenvolvimento folicular ovariano e, após 83 h, uma injeção de gonadotrofina coriônica humana (hCG) (100 UI; IM) para maturação final do oócito e ovulação indução. Trinta e quatro horas após a administração de hCG, 3 × 106 espermatozoides frescos foram utilizados para inseminação pelas técnicas de BIUI (n = 8 gatas) ou UBI (n = 7 gatas), respectivamente. As taxas de gravidez foram de 75,00% (6/8) pela BIUI e 42,85% (3/7) pelo método UBI. O tamanho médio da ninhada foi de 3,0 ± 0,86 para o método BIUI e 2,0 ± 1,0 para o método UBI. Aborto espontâneo ocorreu no dia 35 de gestação em uma gata seguindo o método UBI. Nossos achados mostraram que a BIUI de gatas com sêmen fresco resultou em maiores taxas de prenhez do que o novo método UBI; também, taxas de prenhez aceitáveis foram alcançadas após BIUI com sêmen fresco no gato doméstico.
Downloads
Referências
ACKERMANN CL & LOPES MD. 2020. Training tom cats for semen collection using an artificial vagina: a retrospective study. Journal of feline medicine and surgery 22: 1155-1159.
AZUMANO A et al. 2022. Successful laparoscopic oviductal artificial insemination in the endangered Tsushima leopard cat (Prionailurus bengalensis euptilurus). Animals 12: 777.
CHATDARONG K et al. 2007. Pregnancy in the domestic cat after vaginal or transcervical insemination with fresh and frozen semen. Theriogenology 68: 1326-1333.
CONFORTI VA et al. 2013. Laparoscopic oviductal artificial insemination improves pregnancy success in exogenous gonadotropin-treated domestic cats as a model for endangered felids. Biol Reprod. 89: 4.
DAŞKIN A et al. 2022. Artificial insemination in a cat: Report of first successful performance resulted with parturition in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi 69: 225-227.
FARSTAD W. 2000. Current state in biotechnology in canine and feline reproduction. Animal Reproduction Science 60: 375-387.
FILLIERS M et al. 2010. In vitro evaluation of fresh sperm quality in tomcats: a comparison of two collection techniques. Theriogenology 74: 31-39.
GOODROWE K et al. 1988. Influence of gonadotropins on and developmental competence of in vitro fertilized feline follicular oocytes. Theriogenology 29: 250.
GRAHAM LH et al. 2000. Chorionic gonadotropin administration in domestic cats causes an abnormal endocrine environment that disrupts oviductal embryo transport. Theriogenology 54: 1117-1131.
HOWARD J & WILDT DE. 2009. Approaches and efficacy of artificial insemination in felids and mustelids. Theriogenology 71: 130-148
HOWARD JG et al. 1992. The effect of pre-ovulatory anaesthesia on ovulation in laparoscopically inseminated domestic cats. J Reprod Fertil 96: 175-186.
JOHNSON AK. 2018. Assisted reproduction in the female cat. Veterinary Clinics: Small Animal Practice 48: 523-531.
LERMEN D et al. 2009. Cryobanking of viable biomaterials: implementation of new strategies for conservation purposes. Molecular Ecology 18: 1030-1033.
LINDE-FORSBERG C. 2005. Recent advances in small animal reproduction. Ithaca: International Veterinary.
LUEDERS I et al. 2012. Improved semen collection method for wild felids: urethral catheterization yields high sperm quality in African lions (Panthera leo). Theriogenology 78: 696-701.
PELICAN KM et al. 2001. (Editors). Short-term ovarian suppression with levonorgestrel before gonadotropin stimulation enhances IVF embryo production in the domestic cat. In: MONROE ST & MADISON WI. Biology of Reproduction. USA: SOC STUDY REPRODUCTION.
PELICAN KM et al. 2006. Ovarian control for assisted reproduction in the domestic cat and wild felids. Theriogenology 66: 37-48.
PELICAN KM et al. 2008. Priming with progestin, but not GnRH antagonist, induces a consistent endocrine response to exogenous gonadotropins in induced and spontaneously ovulating cats. Domestic animal endocrinology 34: 160-175.
PLANELLAS M et al. 2012. Mummified fetus in the thoracic cavity of a domestic short-haired cat. Topics in companion animal medicine 27: 36-37.
PLATZ C et al. 1978. Pregnancy in the domestic cat after artificial insemination with previously frozen spermatozoa. Reproduction 52: 279-282.
PROCHOWSKA S et al. 2015. Characteristics of urethral and epididymal semen collected from domestic cats—A retrospective study of 214 cases. Theriogenology 84:1565-1571.
PUKAZHENTHI BS & WILDT DE. 2003. Which reproductive technologies are most relevant to studying, managing and conserving wildlife? Reproduction, Fertility and Development 16: 33-46.
RIJSSELAERE T & VAN SOOM A. 2010. Semen collection, assessment and artificial insemination in the cat. Vlaams Diergeneeskundig Tijdschrift 79: 467-470.
ROTH TL et al. 1997. Effects of equine chorionic gonadotropin, human chorionic gonadotropin, and laparoscopic artificial insemination on embryo, endocrine, and luteal characteristics in the domestic cat. Biology of reproduction 57: 165-171.
SOJKA N et al. 1970. Artificial insemination in the cat (Felis catus L.). Laboratory animal care 20: 198-204.
SWANSON W et al. 1996. Ancillary follicle and secondary corpora lutea formation following exogenous gonadotropin treatment in the domestic cat and effect of passive transfer of gonadotropin-neutralizing antisera. Theriogenology 45: 561-572.
SWANSON WF. 2012. Laparoscopic Oviductal Embryo Transfer and Artificial Insemination in Felids – Challenges, Strategies and Successes. Reproduction in Domestic Animals 47: 136-140.
SWANSON WF. 2006. Application of assisted reproduction for population management in felids: the potential and reality for conservation of small cats. Theriogenology 66: 49-58.
SWANSON WF. 2019. Practical application of laparoscopic oviductal artificial insemination for the propagation of domestic cats and wild felids. Reproduction, Fertility and Development 31: 27-39.
TANAKA A et al. 2000. Artificial intravaginal insemination using fresh semen in cats. J Vet Med Sci. 62: 1163-1167.
TSUTSUI T et al. 2000. Unilateral intrauterine horn insemination of fresh semen in cats. J Vet Med Sci. 62: 1241-1245.
TSUTSUI T et al. 2001. Intratubal insemination with fresh semen in cats. Journal of reproduction and fertility Supplement. 57: 347-351.
TSUTSUI T et al. 2003. Artificial insemination with frozen epididymal sperm in cats. Journal of veterinary medical science 65: 397-399.
TSUTSUI T et al. 2004. Relationship between the sperm count and the fertilization rate of ova ovulated from the contralateral ovary in intrauterine horn insemination in cats. Journal of veterinary medical science 66: 1143-1145.
WATSON PF. 2000. The causes of reduced fertility with cryopreserved semen. Animal reproduction science 60: 481-492.
WILDT DE et al. 1977. Laparoscopy for direct observation of internal organs of the domestic cat and dog. American Journal of Veterinary Research 38:1429-1432.
WILDT DE & SEAGER SW. 1978. Ovarian response in the estrual cat receiving varying dosages of HCG. Hormone Research in Paediatrics 9: 144-150.
WILDT DE. 1991. Fertilization in cats. In: DUNBAR BS & O’RAND MG. (Eds) A comparative overview of mammalian fertilization: Boston:Springer. p. 299-328.
ZAMBELLI D & CASTAGNETTI C. 2001. Transcervical insemination with fresh or frozen semen in the domestic cat: new technique and preliminary results. ESDAR Newsletter 6: 34.
ZAMBELLI D & CUNTO M. 2005. Transcervical artificial insemination in the cat. Theriogenology 64: 698-705.
ZAMBELLI D et al. 2004. Vaginal and cervical anatomic modifications during the oestrus cycle in relation to transcervical catheterization in the domestic cat. Reproduction in domestic animals 39: 76-80.
ZAMBELLI D et al. 2008. Quality and in vitro fertilizing ability of cryopreserved cat spermatozoa obtained by urethral catheterization after medetomidine administration. Theriogenology 69: 485-490.
ZAMBELLI D et al. 2015a. Endoscopic transcervical catheterization in the domestic cat. Reproduction in Domestic Animals 50:13-16.
ZAMBELLI D et al. 2015b. First deliveries after estrus induction using deslorelin and endoscopic transcervical insemination in the queen. Theriogenology 84: 773-778.
Downloads
Publicado
Como Citar
Edição
Seção
Licença
Copyright (c) 2023 Autores e Revista de Ciências Agroveterinárias
Este trabalho está licenciado sob uma licença Creative Commons Attribution-NonCommercial 4.0 International License.
Os autores que publicam nesta revista estão de acordo com os seguintes termos:
a) Os autores mantêm os direitos autorais e concedem à revista os direitos autorais da primeira publicação, de acordo com a Creative Commons Attribution Licence. Todo o conteúdo do periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons do tipo atribuição BY.
b) Autores têm autoridade para assumir contratos adicionais com o conteúdo do manuscrito.
c) Os autores podem fornecer e distribuir o manuscrito publicado por esta revista.