Study on the effect of metabolites secondary of Streptomyces on the bacterium Pseudomonas aeruginosa isolated from dog’s wound
DOI:
https://doi.org/10.5965/223811712342024652Keywords:
Pseudomonas aeruginosa, Streptomyces , secondary metabolitesAbstract
In Baghdad, Iraq, private veterinary clinics provided one hundred samples between February and December 2022. The study included 72 female and 28 male dogs with wound infections. Thirty isolates of P. aeruginosa were identified using biochemical assays and cultural traits. At Tikrit University, ten isolates of Streptomyces bacteria were discovered in soil samples collected from various locations in 2022. Biochemical testing and microscopic analysis were used to identify the isolates. Streptomyces secondary metabolites were subjected to an antibacterial activity assay at doses of 1024, 512, and 256μg/mL. The results illustrated that raw bacterial extract (Streptomyces spp.) exhibited considerable antibacterial activity against all isolates of P. aeruginosa. The results demonstrated that a concentration of 1024μg/mL was more efficient than 256μg/mL in combating P. aeruginosa. The susceptibility test of all P. aeruginosa isolates to 10 antibiotics was carried out by Kirby-Bauer method. The results demonstrated that all isolates were resistant to Ampicillin and Erythromycin (96.66%) followed by Ceftraxone (63.33%), Azithrofomycin (50%), Levofloxacin (46.66%), Cefotaxime, Ciprofloxacin and Gentamycin (43.33%), Ceftaziaime (26.66%) and Amikacin (6.66%). The results of the current study indicated that the majority of isolates with high resistance to antibiotics could be affected by Streptomyces secondary metabolites to expedite the healing of infected wounds.
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References
ABD AH. 2022. Antibacterial and antibiofilm activity of purified Lactobacillus salivarius exopolysaccharide against Pseudomonas aeruginosa. M.Sc. Thesis- in Biology/Microbiology - Mustansiriyah University, Iraq.
ABEDAL-NABIE WG. 2021. Molecular detection of some virulence genes in Pseudomonas aeruginosa Isolation from clinical samples. M.Sc. Thesis- College of Science- Baghdad University, Iraq.
ALFRED EB. 2005. Microbiological Applications in Laboratory Manuals in General Microbiology. 9.ed. McGraw-Hill Company.
AL-AJEELI HRAH. 2012. Biological Effectiveness Determination for Antibiotic Extracts from some Local Streptomyces Isolates. M.Sc. Thesis -Tikrit University, Iraq.
ALHAZMI A. 2015. Pseudomonas aeruginosa: Pathogenesis and pathogenic mechanisms’ International Journal of Biology 7: 44-67.
ALORNAAOUTI AF. 2015. Study of genotyping and some virulence factors of Pseudomonas aeruginosa. Thesis. University of Baghdad- College of Education for Pure Sciences (Ibn-Al-Haitham).
AL SHAMMAA NF. 2016. Virulence genes profile of Pseudomonas aeruginosa local isolates from burns and wounds. Thesis. University of Baghdad- College of Science.
AL ABOOD MAJ. 2022. Investigation of Some Immunological and Molecular Factors Related to Pseudomonas Aeruginosa Caused Burns and Wounds Infection. M.Sc. Thesis- in Genetic Engineering and Biotechnology- Baghdad University, Iraq.
ATLAS RM. 1995. Principles of Microbiology. Mosloy Year Book, Inc. p: 65-69.
Bauer, A.W. and Kirbay, W.A.W.(1966). Antibiotic susceptibility testing by standardized single disc method. Am.J. Clin. Patholo., 45:493-496.
BANIYA A et al. 2018. Isolation and screening of antibiotics producing Streptomyces spp. from the soil collected around the root of Alnus nepalensis from Godawari. Nep. J. Biotech. 6: 46–56.
BECERRIL A et al. 2018. Uncovering production of specialized metabolites by Streptomyces argillaceus: Activation of cryptic biosynthesis gene clusters using nutritional and genetic approaches. PLoS ONE 13: e0198145.
BENSON SAEB. 2005. Microbiology Applications. 9.ed. New York: Laboratory Manual in general.
CHE SOH NA et al. 2020. Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats. Evidence-Based Complementary and Alternative Medicine.
CLSI. 2022. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; M10032h Edition PA: CLSI.
DAVIS BD et al. 1990. Microbiology. 4.Ed. New York: Harper and Row Publishers.
EXNER M et al. 2017. Antibiotic resistance: what is so special about multidrug-resistant gram-negative bacteria? GMS Hyg Infect Control 12: 5.
FORBES BA et al. 2016. Study Guide for Bailey and Scott’s Diagnostic Microbiology-E-Book. Elsevier Health Sciences.
HAYATI M et al. 2019. Molecular detection of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates of chicken origin from East Java, Indonesia. Vet World 12: 578
HOPWOOD DA. 2019. Highlights of Streptomyces genetics. Heredity 123: 23–32.
JOO GJ & RHEE IK. 1997. Production of glucose isomerase from xylb mutant Streptomyces chibensis J- 59. Kor. J. Appl. Microbiol. Biotechnol. 25: 75-81.
KEMPF I et al. 2015. Impact of antibiotic use in the swine industry by Mary D. Barton. Curr Opin Microbiol 19: 137-138.
KORN WF & KUTZNER H. 1992. Family of Streptomycetaceae Prokaryotes. New York: Springer-Verlag. p.921-955.
LEE Y & HWANG K. 2002. Skin thickness of Korean adults. Surgical and radiologic anatomy 24: 183.
MacFADDIN JF. 2000. Biochemical testes for identification of medical bacteria. 3.ed. Pennsylvania: Lippincott Williams and Wilkins.
MAHDI LH et al. 2021. Establishing novel roles of bifidocin LHA, antibacterial, antibiofilm and immunomodulator against Pseudomonas aeruginosa corneal infection model. International Journal of Biological Macromolecules 186: 433-444.
MARQUES DRA. 2015. Prevalência e Susceptibilidade de Isolados Clínicos de Pseudomonas aeruginosa numa unidade hospitalar de Portugal. Ph.D. Thesis. Braga: University of Minho.
MATHUR T et al. 2006. Detection of biofilm formation among the clinical isolates of staphylococci: an evaluation of three different screening methods. Indian Journal of Medical Microbiology 24: 25–29.
NICIEZA RG et al. 1999. Purification characterization and role of nucleases and serine protease in Streptomyces differentiation. Journal of Biology and Chemistry 23: 20366-20375.
NIKBIN VS et al. 2012. Molecular identification and detection of virulence genes among Pseudomonas aeruginosa isolated from different infectious origins. Iranian J Microbiol. 4: 118.
NOOMI BS. 2018. Detection of virulence factors of Pseudomonas aeruginosa in different animals by using bacteriological and molecular methods. Iraqi Journal of veterinary sciences 32: 205-210.
OLIVER A et al. 2015. The increasing threat of Pseudomonas aeruginosa high-risk clones. Drug Resist. Update 2:41–59.
OYEDOH OP et al. 2023. Rare rhizo-Actinomycetes: A new source of agroactive metabolites. Biotechnology Advances: 108205.
QUINN PJ et al. 1998. Clinical Vet Microbiol. Mosby Pub Co.: 237-242.
QUINN PJ et al. 2004. Clinical Veterinary Microbiology. 6.Ed. London: Mosbyimp. Wolf. p. 434-438.
RAHEEM SAK. 2014. Study the effect of bacterial extracts of filamentous bacteria (Streptomyces chromofucus NS-38) on the bacteriam Staphylococcus aureus isolated from cases of mastitis in cows. M.Sc. Thesis- College of Veterinary Medicine- Baghdad University, Iraq.
RAJARAM SK et al. 2020. Extraction and purification of an antimicrobial bioactive element fromlichen associated Streptomyces olivaceus LEP7 against wound inhabitingmicrobial pathogens. JKSUS 32: 2009–2015.
RIAZ-RAJOKA MS et al. 2020. Lactobacillus exopolysaccharides: new perspectives on engineering strategies, physiochemical functions, and immunomodulatory effects on host health. Trends Food Sci. Technol. 103: 36–48.
ROSENTHAL VD et al. 2016. International Nosocomial Infection Control Consortium report, data summary of 50 countries for 2010-2015.Device-associated module. American journal of infection control 44: 1495–1504.
SABARATNAM S & TRAQUAIR JA. 2002. Formulation of a Streptomyces biocontrol agent for the suppression of Rhizoctonia damping-off in tomato transplants. Biological Control 23: 245–253.
SCHROEDER M et al. 2017. The Complex Relationship between Virulence and Antibiotic Resistance. Genes 8: 39.
SHAEBTH LJ. 2018. Molecular identification and sequencing of Pseudomonas aeruginosa virulence genes among different isolates in Al-Diwaneyah hospital. Iraqi Journal of Veterinary Sciences 32: 183-188.
SHEHAB SK. & JASSIM EH. 2019. Impact of Mint oil and Colistin antibiotic on pilB gene of clinical Pseudomonas aeruginosa Isolates from Baghdad, Iraq. Iraqi journal of biotechnology 18: 43-54.
VINGOPOULOU EI et al. 2018. Prevalence and mechanisms of resistance to fluoroquinolones in Pseudomonas aeruginosa 213: 102-107.
WAKSMAN SA. 1976. The Actinomycetes: a summary of current knowledge. New York: The Roland press Co. p.320-325.
WASAN AD. 2021. Wei-ji: Every crisis is an opportunity. Pain Medicine 22: 774-775.
WILLIAM F & PAUL RJ. 2006. Developing a new resource for drug discovery: Marine actinomycetes bacteria. Nat. Chem. Biol.2: 666-673.
WU F et al. 2019. Molecular characterization of a multidrug-resistant Klebsiella pneumoniae strain R46 isolated from a rabbit. Intl J Genom 2019: 5459190.
ZHANG M et al. 2000. A functional NSP4 enterotoxin peptide secreted from rotavirus-infected cells J. Virol. 74: 11663-11670.
ZHANG H et al. 2022.Positive regulation of the MarR-type regulator slnO and improvement of salinomycin production by Streptomyces albus by multiple transcriptional regulation. Can.J. Microbiol. 68: 157–163.
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