COMPARISON OF ANTIGENS USED FOR THE DIAGNOSIS OF BRUCELLA OVIS

Authors

  • Priscilla Karina Vitor Koerich
  • Adil Knackfuss Vaz

DOI:

https://doi.org/10.5965/223811710122002120

Keywords:

Brucella ovis, diagnosis, antigen omposion.

Abstract

Radial Immunodiffusion (RID) is the most widely used diagnostic method for sheep brucellosis due to Brucella ovis. It is also used for the diagnosis of canine brucellosis caused by Brucella canis. Antigens used for RID are not standardized and it is possible that reagents from different sources may produce discrepancies, even if the same bacterial strain is used to produce the antigens. Antigens produced by two laboratories (antigens I, IIa and IIb) were compared to an antigen produced following the techniques recommended by FAO/WHO, and considered as standard for the purposes of this study. Antigens were compared by PAGE analysis and RID, and protein concentration determined by the SENSIPROT technique. It was found that one antigen produced two precipitation lines in RID. The same product showed a different pattern of bands in PAGE, with an extra band of protein at 200 KDa. The same antigen had higher protein concentration than the standard and the other antigens.

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References

CARRILLO, C.G. Conceptos sobre control de la Brucelosis; Gaceta Veterinaria p. 266, 1972.

CHIN, J. C., PANG-TURNER, B. Journal of Clinical Microbiology, Immunology Section v. 28 n. 1, p. 22647- 2656, 1990.

COGGINS, L., PATTEN, V. Immunodiffusion test for Equine Infectious Anemia. Reprinted from PROCEEDINGS OF 74TH ANNUAL MEETING U.S. ANIMAL HEALTH ASSOC., p. 568-571, 1979.

CORBEL, M.J. & BRINLEY-MORGAN, W.J. Genus Brucella (Meyer and Shaw 1920). In N. R. Krieg e J. G. Holt (ed.), BERGEY'S MANUAL OF SYSTEMATIC BACTERIOLOGY, v. 1, p. 377-387. The Williams & Wilkins Co., Baltimore, 1984.

DILENA B.A, PENBERTHY L.A, FRASER CG. Six methods for determining urinary protein compared. CLINICAL CHEMISTRY v. 29 n. 3, p. 553-7, 1983.

GENETZKY, R. M. Epididymitis in Rams. COMPENDIUM ON CONTINUING EDUCATION FOR THE PRACTICING VETERINARIAN v. 17 n. 3, p. 447- 451, 1970

LAEMMLI, U. K. Cleavage of structural proteins during assembly of the head of bacteriophage T4; NATURE n. 227, p. 680-685, 1970.

McELDERRY L.A, TARBITT I.F, CASSELLS - SMITH A.J. Six methods for urinary protein compared. CLINICAL CHEMISTRY V. 28 n. 2, p. 356-360, 1982.

RIEZU-BOJ, J. I.; MORIYÓN, I.; BLASCO, J. M.; GAMAZO, C. DIAZ, R. Antibody response to Brucellaovis outer membrane proteins in ovine brucellosis. INFECTION AND IMMUNITY v. 58 n. 2, p. 489-494, 1990.

WESTGARD J.O, GROTH T. Design and evaluation of statistical control procedures: applications of a computer "quality control simulator" program. CLINICAL CHEMISTRY v. 27 n. 9, p. 1536-1545, 1981.

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Published

2002-10-15

How to Cite

KOERICH, Priscilla Karina Vitor; VAZ, Adil Knackfuss. COMPARISON OF ANTIGENS USED FOR THE DIAGNOSIS OF BRUCELLA OVIS. Revista de Ciências Agroveterinárias, Lages, v. 1, n. 2, p. 120–124, 2002. DOI: 10.5965/223811710122002120. Disponível em: https://periodicos.udesc.br/index.php/agroveterinaria/article/view/23927. Acesso em: 11 may. 2024.

Issue

Vol. 1 No. 2 (2002)

Section

Research Article - Science of Animals and Derived Products

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