Plant bioregulators on common bean cultivated under two soil moisture
Keywords:abscisic acid, methyl jasmonate, tropical agriculture, drought stress.
Restrictions on water available lead to possible damages in the bean crop, and the use of bioregulators can improve the plant signal and boost tolerance against drought stress. This study aimed to characterize physiological, biochemical, and biometric alterations in bean plants subjected to foliar application of bioregulators under different soil water levels. The following treatments were applied: control (water) and three bioregulators (5 μM of abscisic acid - ABA, 50 μM of methyl jasmonate – MeJA, and 5 μM of ABA + 50 μM of MeJA). Also, the plants were subjected to two soil water levels (60 and 80% of field capacity), in a 4 x 2 factorial scheme. The variables studied were: content of photosynthetic pigments, leaf gas exchange, and biometric indexes in the common bean plants. Our results showed that the ABA + MeJA treatment increased the concentration of chlorophyll b, ascorbate peroxidase (APX), and catalase (CAT) activities. At the same time, the photosynthetic rate was stimulated by the mixture of bioregulators on 36 days of planting (DAP). ABA and MeJA, applied isolated, caused a higher photosynthetic rate in the bean in the 34 DAP, whereas for the biometric variables, they were only influenced by the soil water levels. Regarding the biochemical mechanisms, it was verified that the ABA + MeJA treatment increased the activity of antioxidant enzymes and improved gas exchange responses in a condition of lower water availability. The bioregulators assessed in this research are beneficial in the modulation of plant physiology in plants under drought stress. However, the adequate water supply represents a better development of the plants.
AHMAD P et al. 2016. Jasmonates: Multifunctional Roles in Stress Tolerance. Frontiers in Plant Science 7: 1-15.
ANDERSON MD et al. 1995. Changes in isozyme profiles of catalase, peroxidase, and glutathione reductase during acclimation to chilling in mesocotyl of maize seedlings. Plant Physiology 109: 1247-1257.
ANJUM SA et al. 2011. Effect of exogenous methyl jasmonate on growth, gas exchange and chlorophyll contents of soybean subjected to drought. African Journal of Biotechnology 10: 9640-9646.
BARBOSA MR et al. 2014. Geração e desintoxicação enzimática de espécies reativas de oxigênio em plantas. Ciência Rural 44: 453-460.
BARI R & JONES JDG. 2009. Role of plant hormones in plant defence responses. Plant Molecular and Biolgy 69:473-488.
BARICKMAN TC et al. 2014. Abscisic acid increases carotenoid and chlorophyll concentrations in leaves and fruit of two tomato genotypes. Journal of the American Society for Horticultural Science 139: 261-266.
BARNABÁS B et al. 2008. The effect of drought and heat stress on reproductive processes in cereals. Plant, Cell and Environment 31: 11-38.
BEAUCHAMP C & FRIDOVICH I. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44: 276-287.
BRADFORD MM. 1976. Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding. Analytical Biochemistry 72: 248-254.
CAVERZAN A et al. 2012. Plant responses to stresses: Role of ascorbate peroxidase in the antioxidant protection. Genetics and Molecular Biology 35: 1011-1019.
CHOUDHARY R et al. 2012. Effect of abscisic acid and hydrogen peroxide on antioxidant enzymes in Syzygium cumini plant. Journal of Food Science and Technology 49: 649-652.
FERNANDES B & SYKES DJ. 1968. Capacidade de campo e retenção de água em três solos de Minas Gerais. Revista Ceres 15: 1-39.
GIANNAKOULA AE et al. 2012. The effects of plant growth regulators on growth, yield, and phenolic profile of lentil plants. Journal of Food Composition and Analysis 28: 46-53.
GIANNOPOLITIS CN & RIES SK. 1977. Superoxide dismutases. I. Occurrence in higher plants. Plant Physiology 59: 309-314.
GILL SS et al. 2015. Superoxide dismutase-mentor of abiotic stress tolerance in crop plants. Environmental Science and Pollution Research 22: 10375-10394.
GRAFTON RQ et al. 2015. Food and water gaps to 2050: preliminary results from the global food and water system (GFWS) platform. Food Security 7: 209-220.
HAVIR EA & McHALE NA. 1987. Biochemical and developmental characterization of multiple forms of catalase in tobacco leaves. Plant Physiology 84: 450-455.
HORN FL et al. 2000. Avaliação de espaçamentos e populações de plantas de feijão visando à colheita mecanizada direta. Pesquisa Agropecuária Brasileira 35: 41-46.
HUANG H et al. 2017. Jasmonate action in plant growth and development. Journal of Experimental Botany 68: 1349-1359.
JALEEL CA. et al. 2009. Drought stress in plants: a review on morphological characteristics and pigments composition. International Journal of Agriculture and Biology 11: 100-105.
JANOUDI A & FLORE JA. 2003. Effects of multiple applications of methyl jasmonate on fruit ripening, leaf gas exchange and vegetative growth in fruit trees. Journal of Horticultural Science & Biotechnology 78: 793-797.
KHADRI M et al. 2006. Alleviation of salt stress in common bean (Phaseolus vulgaris) by exogenous abscisic Acid supply. Journal of Plant Growth Regulation 25: 110-119.
KOBAYAKAWA H & IMAI K. 2012. Methyl jasmonate affects O3-inhibition of photosynthesis and ascorbic acid content in paddy rice grown at different CO2 concentrations. Environmental Control in Biology 50: 335-345.
LANNA AC et al. 2016. Physiological characterization of common bean (Phaseolus vulgaris L.) genotypes, water-stress induced with contrasting response towards drought. Australian Journal of Crop Science 10: 1-6.
LICHTENTHALER H & WELLBURN A. 1993. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions 11: 591-592.
MA C et al. 2014. Photosynthetic responses of wheat (Triticum aestivum L.) to combined effects of drought and exogenous methyl jasmonate. Photosynthetica 52: 377-385.
MACEDO WR et al. 2013. Unravelling the physiologic and metabolic action of thiamethoxan on rice plants. Pesticide Biochemistry and Physiology 107: 244-249.
McLAREN JS & SMITH H. 1976. The effect of abscisic acid on growth, photosynthetic rate and carbohydrate metabolism in Lemina minor. New Phytologist 76: 11-20.
MUÑOZ-PEREA CG et al. 2006. Selection for drought resistence in dry bean landraces and cultivars. Crop Science 46: 2111-2120.
NAKANO Y & ASADA K. 1981. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant and Cell Physiology 22: 867-880.
OHASHI Y et al. 2006. Effects of drought stress on photosynthetic gas exchange, chlorophyll fluorescence and stem diameter of soybean plants. Biologia Plantarum 50: 138-141.
OLIVEIRA AD et al. 2005. Condutância estomática como indicador de estresse hídrico em feijão. Engenharia Agrícola 25: 86-95.
OLIVEIRA MB et al. 2015. Exogenous application of methyl jasmonate induces a defense response and resistance against Sclerotinia sclerotiorum in dry bean plants. Journal of Plant Physiology 182: 13-22.
OSTER U et al. 2000. Cloning and functional expression of the gene encoding the key enzyme for chlorophyll b biosynthesis (CAO) from Arabidopsis thaliana. Plant Journal 21: 305-310.
PAVLOVIĆ D et al. 2014. Chlorophyll as a measure of plant health: Agroecological aspects. Journal Pesticides and Phytomedicine 29: 21-34.
RAHMAN MH. 2016. Exploring sustainability to feed the world in 2050. Journal of Food Microbiology 1: 7-16.
RATNAKUMAR P et al. 2016. Can plant bio-regulators minimize crop productivity losses caused by drought, salinity and heat stress? An integrated review. Journal of Applied Botany and Food Quality 89: 113-125.
SAH SK et al. 2016. Abscisic acid and abiotic stress tolerance in crop plants. Frontiers in Plant Science 7: 571.
SANKAR B et al. 2013. Photosynthetic pigment content alterations in Arachis hypogaea L. in relation to varied irrigation levels with growth hormone and triazoles. Journal of Ecobiotechnology 5: 7-13.
SILVA FG et al. 2015. Trocas gasosas e fluorescência da clorofila em plantas de berinjela sob lâminas de irrigação. Revista Brasileira de Engenharia Agrícola e Ambiental 19: 946-952.
SINGH SP. 1989. Patterns of Variation in Cultivated Common Bean (Phaseolus vulgaris, Fabaceae). Economic Botany 43: 39-57.
SOARES AMS et al. 2010. T. Effect of methyl jasmonate on antioxidative enzyme activities and on the contents of ROS and H2O2 in Ricinus communis leaves. Brazilian Journal of Plant Physiology 22: 151-158.
SUHITA D et al. 2003. Different signaling pathways involved during the suppression of stomatal opening by methyl jasmonate or abscisic acid. Plant Science 164: 481-488.
WASTERNACK C & HAUSE B. 2002. Jasmonates and octadecanoids: signals in plant stress responses and development. Progress in Nucleic Acid Research and Molecular Biology 72: 165-221.
WEBBER HA et al. 2006. Water use efficiency of common bean and green gram grown using alternate furrow and deficit irrigation. Agricultural Water Management 86: 259-268.
WITHAM FH et al. 1971. Experiments in Plant Physiology. New York: D. Van Nostrand Company. p 55-58.
YANG Z et al. 2016. Leveraging abscisic acid receptors for efficient water use in Arabidopsis. Proceedings of the National Academy of Sciences 113: 6791-6796.
ZHAO N et al. 2017. Interaction of CO2 concentrations and water stress in semiarid plants causes diverging response in instantaneous water use efficiency and carbon isotope composition. Biogeosciences 14: 3431-3444.
ZLATEV Z & LIDON FC. 2012. An overview on drought induced changes in plant growth, water relations and photosynthesis. Emirates Journal of Food and Agriculture 24: 57-72.
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