Pregnancy modulates serum proteins, hematological and blood biochemical variables of the Pêga donkeyal variables of the Pêga donkey

Authors

  • Annita Morais Girardi Universidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal http://orcid.org/0000-0003-1370-0711
  • Carmen Zilda Pereira de Toledo Universidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal
  • Renata Lemos Nagib Jorge Universidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal
  • José Jurandir Fagliari Universidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal
  • Amanda Festa Sabes Universidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal
  • Luiz Carlos Marques Universidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal

DOI:

https://doi.org/10.5965/223811711732018377

Keywords:

acute phase protein, biochemistry, electrophoresis, Equidae, gestation, hematology

Abstract

Considering the scarcity of information regarding blood parameters of Pêga donkeys (Equus asinus), and the influence of factors such as breed and physiological status on these parameters, this study aimed to evaluate the influence of pregnancy on hematological, blood biochemical and serum protein profiles of the Pêga donkey, using samples from 59 female adult donkeys, 22 pregnant and 37 non-pregnant, kept under field conditions. The parameters measured were counts of red blood cells (RBC), white blood cells (WBC), basophils, eosinophils, bands, segmented neutrophils, lymphocytes, monocytes and platelets; and the levels of packed cell volume, hemoglobin concentration, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatine kinase, gamma-glutamyl transferase, urea, glucose, cholesterol, triglycerides, total calcium, phosphorus, magnesium, chlorides, sodium, potassium, ionized calcium, creatinine, total protein, albumin, total, direct and indirect serum bilirubin. Serum protein fractionation was performed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), which found 10 proteins: immunoglobulins A and G, ceruloplasmin, transferrin, albumin, haptoglobin, α1-acid glycoprotein, 138 kDa (MWP138), 33 kDa (MWP33) and 23 kDa (MWP23) molecular weight proteins. RBC and triglycerides were higher in pregnant females. Urea, Immunoglobulin A, MWP138 and MWP23 concentrations were higher in non-pregnant mares. This study presented differences from previous studies, and some differences in blood variables between pregnant and non-pregnant Pêga donkeys, confirming that pregnancy causes significant metabolic changes, which must be considered in the clinical evaluation of these animals.


Downloads

Download data is not yet available.

References

AMINO N et al. 1978. Changes of serum immunoglobulins IgG, IgA, IgM, and IgE during pregnancy. Obstetrics & Gynecology 52: 415-420.

BONELLI F et al. 2016. Hematological and biochemical findings in pregnant, postfoaling, and lactating jennies. Theriogenology 85: 1233-1238.

CAMPOS JM et al. 1968. Hemograma do jumento Pêga. Arquivos da Escola Superior de Veterinária do Estado de Minas Gerais 20 :55-58.

CANISSO IF & MCDONNELL SM. 2010. Donkey breeding behavior with an emphasis on the Pêga breed. In: MATTHEWS NS &TAYLOR TS. Veterinary Care of Donkeys. Ithaca: International Veterinary Information Service. No. A2926.0310. Available at: http://www.ivis.org/advances/Matthews/canisso/chapter.asp?LA=1. Access on: Apr. 30, 2017.

ECKERSALL PD. 2008. Proteins, proteomics, and the dysproteinemias. In: KANEKO JJ et al. Clinical Biochemistry of Domestic Animals. 6.ed. Boston: Elsevier Academic Press. p.117-155

FAZIO E et al. 2011. Changes in the cortisol and some biochemical patterns of pregnant and barren jennies (Equus asinus). Veterinarski Arhiv 81: 563-574.

FORHEAD AJ et al. 1994. Endocrine and metabolic responses to transportation in periparturient donkeys. Animal Reproduction Science 36: 315-327.

GACEK F et al. 1977. Evolution of blood serum globulins as determined by pregnancy in the domestic donkey (Equus asinus L.). British Veterinary Journal 133: 548-553.

GEFFRE A et al. 2011. Reference Value Advisor: a new freeware set of macroinstructions to calculate reference intervals with Microsoft Excel.Veterinary Clinical Pathology 40: 107-112.

GIGUÈRE S & POLKES AC. 2005. Immunologic disorders in neonatal foals. Veterinary Clinics of North America: Equine Practice 21: 241-272.

GIRARDI AM et al. 2014. Biochemical profile of the Pêga donkey (Equus asinus) breed: influence of age and sex. Comparative Clinical Pathology 23: 941-947.

GIRARDI AM et al. 2015. Hematological variables of the Pêga donkey (Equus asinus) breed: influence of age and sex. Comparative Clinical Pathology 24: 335-342.

GIRARDI AM et al. 2016. Serum protein fractions, hematological and biochemical variables of the Pêga donkey (Equus asinus) breed in the first year of age. Veterinary Archives 86: 335-349.

GIRARDI AM et al. 2017. The effect of age and sex on serum proteins in the Pega donkey (Equus asinus). Veterinarni Medicina 62:10-15.

GRAVENA K et al. 2010. Parâmetros hematológicos de jumentas gestantes em diferentes períodos. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 62: 1514-1516.

GROSSMAN CJ. 1985. Interactions between the gonadal steroids and the immune system. Science 227: 257-261.

JAIN NC. 1993. Essentials of veterinary hematology. Philadelphia: Lea & Febiger. 417p.

LAEMMLI U. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.

MORI E et al. 2004. Reference values on hematologic parametes of the Brazilian donkey (Equus asinus) breed. Journal of Equine Veterinary Science 24: 271-276.

MURATA H et al. 2004. Current research on acute phase proteins in veterinary diagnosis: an overview. The Veterinary Journal 168: 28-40.

PERDIGÃO DE OLIVEIRA FRA et al. 1983. Perfil das globulinas do soro sangüíneo, durante a gestação, na jumenta. Boletim de Indústria Animal 40: 219-227

ROSENFELD G. 1947. Corante pancrômico para hematologia e citologia clínica. Nova combinação dos componentes do May-Grunwald e do Giemsa num só corante de emprego rápido. Memórias do Instituto Butantan 20: 329-335

SOUZA AI et al. 2002. Alterações hematológicas e gravidez. Revista Brasileira de Hematologia e Hemoterapia 24: 29-36.

TOLEDO CZP et al. 2015. Morfologia da placenta a termo e da interface materno-fetal da jumenta Pêga. Brazilian Journal of Veterinary Research and Animal Science 52: 195-204.

TUNÓN AM et al. 1998. Concentrations of total protein, albumin and immunoglobulins in undiluted uterine fluid of gynecologically healthy mares. Theriogenology 50: 821-831.

VERONESI MC et al. 2014. IgG, IgA, and lysozyme in Martina Franca donkey jennies and their foals. Theriogenology 81: 825-831.

YASUHARA M et al. 1992. Reciprocal changes in serum levels of immunoglobulins (IgG, IgA, IgM) and complements (C3, C4) in normal pregnancy and after delivery. Journal of Clinical & Laboratory Immunology 38: 137-141.

Downloads

Published

2018-09-26

How to Cite

GIRARDI, Annita Morais; TOLEDO, Carmen Zilda Pereira de; JORGE, Renata Lemos Nagib; FAGLIARI, José Jurandir; SABES, Amanda Festa; MARQUES, Luiz Carlos. Pregnancy modulates serum proteins, hematological and blood biochemical variables of the Pêga donkeyal variables of the Pêga donkey. Revista de Ciências Agroveterinárias, Lages, v. 17, n. 3, p. 377–382, 2018. DOI: 10.5965/223811711732018377. Disponível em: https://periodicos.udesc.br/index.php/agroveterinaria/article/view/9617. Acesso em: 22 dec. 2024.

Issue

Vol. 17 No. 3 (2018)

Section

Research Article - Science of Animals and Derived Products

License

Copyright (c) 2018 Revista de Ciências Agroveterinárias (Journal of Agroveterinary Sciences)

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors publishing in this journal are in agreement with the following terms:

a) Authors maintain the copyrights and concede to the journal the copyright for the first publication, according to Creative Commons Attribution Licence.

b) Authors have the authority to assume additional contracts with the content of the manuscript.

c) Authors may supply and distribute the manuscript published by this journal.