Protocol for micropropagation of quince BA29 in semisolid media

Authors

  • Fernanda Grimaldi Centro de Ciências Agroveterinárias - UDESC
  • Aline Meneguzzi Centro de Ciências Agroveterinárias - UDESC
  • Gabriela Candido Weber Centro de Ciências Agroveterinárias - UDESC
  • Daiane Corrêa Centro de Ciências Agroveterinárias - UDESC
  • Mayra Juline Gonçalves Centro de Ciências Agroveterinárias - UDESC
  • Leo Rufato Centro de Ciências Agroveterinárias - UDESC
  • Aike Anneliese Kretzschmar Centro de Ciências Agroveterinárias - UDESC

DOI:

https://doi.org/10.5965/223811711532016266

Keywords:

In vitro propagation, Cydonia oblonga, growth regulators.

Abstract

Micropropagation is a technique widely known for its ability to produce large numbers of plants in a short period of time. It is essential for breeding programs and to establish micropropagation protocols for economically important species. The aim of this study was to establish a micropropagation protocol for quince BA29, aiming at mass production of plantlets. The explants of quince BA29 were cultured on MS and WPM media (original and 1/2) in combination with BAP (0, 0.5, 1, 2 and 4 mg L-1) for the in vitro multiplication and cultured in MS medium with sucrose (15 and 30 mg.L-1) combined with IBA (0, 0.12, 0.25, 0.50, 1.0 and 2.0 mg L-1) for in vitro rooting. After 45 days, the culture media which provided the best results for growth and multiplication of BA29 explants was the MS original with 2.68 mg L-1 of BAP and the culture media which provided the best results for rooting of BA29 explants was MS with 15 g L-1 of sucrose and 1.25 mg L-1 of IBA.

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Published

2016-11-11

How to Cite

GRIMALDI, Fernanda; MENEGUZZI, Aline; WEBER, Gabriela Candido; CORRÊA, Daiane; GONÇALVES, Mayra Juline; RUFATO, Leo; KRETZSCHMAR, Aike Anneliese. Protocol for micropropagation of quince BA29 in semisolid media. Revista de Ciências Agroveterinárias, Lages, v. 15, n. 3, p. 266–270, 2016. DOI: 10.5965/223811711532016266. Disponível em: https://periodicos.udesc.br/index.php/agroveterinaria/article/view/223811711532016266. Acesso em: 22 dec. 2024.

Issue

Vol. 15 No. 3 (2016)

Section

Scientific Note

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