Transferability of microssatellite markers to Trichogramma pretiosum Riley, 1879 (Hymenoptera: Trichogrammatidae)

Authors

DOI:

https://doi.org/10.5965/223811712112022066

Keywords:

Biological control, Genetic variation, Molecular markers, Parasitoid wasp

Abstract

The biological pest control has expanded in Brazil with the Trichogramma pretiosum as the main natural enemy. The microsatellite molecular markers Simple Sequence Repeat (SSR) have been the most used, as they are multiallelic, robust and reproducible, in several species. In order to optimize future processes of identification and analysis of the parasitoid's genetic diversity, twenty markers, isolated and characterized for the parasitoid wasp Trichogramma dendrolimi, were tested in 15 generations of T. pretiosum. Those markers, ten have been transferred and can be used to evaluate the genetic variation of T. pretiosum.

 

Downloads

Download data is not yet available.

References

BARBOSA MG et al. 2021. Do fall armyworm’s Metaflumizone resistante populations affect the activity of Trichogramma pretiosum? Brazilian Journal of Biology 1: 1-6.

BORBA RS et al. 2005. Dissimilaridade genética de linhagens de Trichogramma Westwood (Hymenoptera: Trichogrammatidae) através de marcadores moleculares ISSR. Neotropical Entomology 34: 565-569.

CHAPUIS M-P & ESTOUP A. 2007. Microsatellite null alleles and estimation of population differentiation. Molecular Biology and Evolution 24: 621-631.

FRAGOSO DFM et al. 2020. Trichogramma Westwood, 1833 (Hymenoptera, Trichogrammatidae) collected in orchards in Rio Grande do Sul State, Brazil. Acta Biológica Catarinense 4: 50-57.

LITT M & LUTY JA. 1989. A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. American Journal of Human Genetics 44: 397-401.

LÜ X & HAN S. 2016. Isolation and characterization of 26 polymorphic microsatellite markers for the parasitoid wasp Trichogramma dendrolimi (Hymenoptera: Trichogrammatidae). Biocontrol Science and Technology 1: 1-11.

OLERUP O & ZETTERQUIST H. 1994. HLA-DR typing by PCR amplification witch sequence specific primer (PCR-SSP) in 2 hours: An alternative to serological DR typing in clinical practice including donor-repient matching in cadaveric transplantation. Tissue Antigens 39: 225-235.

REGITANO LCA. 2007. Quantificação em espectrofotômetro. In: REGITANO LCA et al. (Org.). Protocolos em biologia molecular aplicada à produção animal. São Carlos: Embrapa Pecuária Sudeste. p.12-13.

SANTOS SKS et al. 2020. Transferibilidade de marcadores SSR, diversidade e estrutura genética em Syagrus oleracea. Brazilian Journal of Development 8: 59931-59947.

SCHLÖTTERER C & TAUTZ D. 1992. Slippage synthesis of simple sequence DNA. Nucleic Acids Research 20: 211-215.

WEBER JL & MAY PE. 1989. Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. American Journal of Human Genetics 44: 388-396.

ZORTÉA KEM et al. 2021. Transferability from microsatellites from Vochysia ferruginea Mart. to Vochysia divergens Pohl. Brazilian Journal of Development 3: 24014-24026.

Downloads

Published

2022-03-04

How to Cite

ARAÚJO JÚNIOR , Luis Moreira de; ZAGO, Hugo Bolsoni; FERREIRA, Marcia Flores da Silva; PRATISSOLI, Dirceu; PEREIRA, Aléxia Gonçalves; DAMASCENA, Alixelhe Pacheco. Transferability of microssatellite markers to Trichogramma pretiosum Riley, 1879 (Hymenoptera: Trichogrammatidae). Revista de Ciências Agroveterinárias, Lages, v. 21, n. 1, p. 66–70, 2022. DOI: 10.5965/223811712112022066. Disponível em: https://periodicos.udesc.br/index.php/agroveterinaria/article/view/20884. Acesso em: 30 jun. 2024.

Issue

Section

Research Note - Science of Animals and Derived Products